Question: You Are Interested In Expressing The Pax6 Master Regulator For Eye Development In Ectopic (not Natural) Locations. You Observe: • 100 Copies Of The Pax6 Coding Region Under The Control Of The Normal Pax6 Transcriptional Regulatory Sequences >> HAS Ectopic Eye Development. • 8 Copies Of The Pax6 Coding Region Under The Control Of The Normal Pax6 …

Question: You Are Interested In Expressing The Pax6 Master Regulator For Eye Development In Ectopic (not Natural) Locations. You Observe: • 100 Copies Of The Pax6 Coding Region Under The Control Of The Normal Pax6 Transcriptional Regulatory Sequences >> HAS Ectopic Eye Development. • 8 Copies Of The Pax6 Coding Region Under The Control Of The Normal Pax6 …

You are interested in expressing the Pax6 master regulator foreye development in ectopic (not natural) locations.  Youobserve:

• 100 copies of the Pax6 coding region under the control of thenormal Pax6 transcriptional regulatory sequences >> HASectopic eye development.

• 8 copies of the Pax6 coding region under the control of thenormal Pax6 transcriptional regulatory sequences >> NOectopic eye development.

• 8 copies of Pax6 coding region under the control of thetranscriptional regulatory sequence for another gene (gene X)>> HAS ectopic eye development.  Note that the theregulatory sequence for gene X has the same level of expression asthat of the normal Pax6 regulatory sequences when Pax6 is expressednormally in the eye.

Describe the likely scenario generating the different outcomeswhen 8 copies are utilized under the different transcriptionalregulatory controls.  Include in your answer, adescription of the basic principle of how gene regulatory networksspecify tissue development, and how the two scenarios would beimpacted under this scenario, i.e., why there are differentoutcomes for 8 copies of the normal Pax 6 regulatory sequence vs. 8copies of the gene X regulatory sequence.